东北粳稻分蘖期耐冷性鉴定及SSR标记关联分析

doi:10.3969/j.issn.10017216.2012.04.006

摘要/Abstract

摘要： 以140份东北粳稻品种（系）为材料进行分蘖期耐冷鉴定，用84个SSR标记对耐冷性差异较大的96份材料进行基于SSR标记的关联分析。结果表明，供试材料强耐冷品种（系）占20%，中间型（系）占30.71%，不耐冷品种（系）占49.28%；分蘖期耐冷性与结实率和单株穗重呈极显著正相关，与生育期呈极显著负相关；关联分析得到18个位点与分蘖期耐冷性关联，其中标记RM214，RM445，RM506，RM106，RM215，RM182，RM270和RM229与前人研究获得的水稻耐冷性QTL定位结果吻合。这些标记可作为东北粳稻耐冷性资源筛选重点选择的标记。还通过优异等位基因的挖掘，得到一系列的优异等位变异及载体材料。

关键词: 粳稻, 分蘖期, 耐冷性, 关联分析

Abstract: The cold tolerance of rice at the tillering stage was evaluated with 140 rice cultivars(lines) from Northeast China as materials and association analysis was conducted among 96 selected materials which differed in cold tolerance with 84 SSR markers. The experimental results showed: there were 20.0% of rice materials with better cold tolerance, 30.71% with intermediate cold tolerance and 49.28% with worse cold tolerance; Correlation analysis showed that rice cold tolerance at the tillering stage was significantly positively correlated with seed setting rate and single panicle weight. Rice cold tolerance at the tillering stage and growth duration were significantly negatively correlated; Eighteen markers associated with rice cold tolerance were detected through the association analysis, markers like RM214，RM445，RM506，RM106，RM215，RM182，RM270 and RM229 were mutual authentication with familybased linkage mapping. These markers can be used for selection of rice cold tolerance resources in Northeast China. Finally, a serial of elite alleles, loci and their carrier materials were screened out by anglicizing these associated markers.

Key words: japonica, tillering stage, cold tolerance, association analysis

中图分类号:

张艳梅,邹德堂*. 东北粳稻分蘖期耐冷性鉴定及SSR标记关联分析[J]. 中国水稻科学, 2012, 26(4): 423-430.

ZHANG Yanmei, ZOU Detang* . Association Analysis of Rice Cold Tolerance at Tillering Stage with SSR Markers in japonica Cultivars in Northeast China[J]. Chinese Journal of Rice Science, 2012, 26(4): 423-430.

参考文献

\[1\]郭晓丽, 王立刚, 邱建军，等.基于GIS的东北地区水稻低温冷害区划研究. 江西农业大学学报, 2009, 31（3）: 494498.

\[2\]崔锦, 李辑, 张爱忠, 等.东北夏季低温的研究进展.气象, 2007, 33(4): 39.

\[3\]姚佩珍.近四十年东北夏季低温冷害的气候特征.灾害学, 1995, 10(1): 5256.

\[4\]Baruah A R, IshigoOka N, Adachi M, et al. Cold tolerance at the early growth stage in wild and cultivated rice. Euphytica, 2009, 165(3): 459470.

\[5\]Zhou L, Zeng Y W, Zheng W W, et al. Fine mapping a QTL qCTB7for cold tolerance at the booting stage on rice chromosome 7 using a nearisogenic line. Theor Appl Genet, 2010, 121(5): 895905.

\[6\]Suh J P, Jeung J U, Lee J I , et al. Identification and analysis of QTLs controlling cold tolerance at the reproductive stage and validation of effective QTLs in coldtolerant genotypes of rice (Oryza sativa L.). Theor Appl Genet, 2010, 120(5): 985995.

\[7\]Saito K, HayanoSaito Y, Kuroki M, et al. Mapbased cloning of the rice cold tolerance gene Ctb1. Plant Sci, 2010, 179(112): 97102.

\[8\]韩龙植, 乔永利, 曹桂兰, 等.水稻生长早期耐冷性QTL分析. 中国水稻科学, 2005, 19(2): 124125.

\[9\]Zhu Y L, Song Q J, Hyten D L, et al. Single nucleotide polymorphisms in soybean. Genetics, 2003, 163（3）: 11231134.

\[10\]王荣焕, 王天宇, 黎裕. 关联分析在作物种质资源分子评价中的应用. 植物遗传资源学报, 2007, 8(3): 366372.

\[11\]何静, 王文泉, 郑永清, 等.木薯栽培品种农艺性状与分子标记的关联分析. 热带作物学报, 2010, 31(5): 693698.

\[12\] Jin L, Bao J S. Progress on the traitmarker association analysis in plants. Mol Plant Breed, 2009, 7(6): 10481063

\[13\]Wen W, Mei H,Feng F,et al. Population structure and association mapping on chromosome 7 using a diverse panel of Chinese germplasm of rice (Oryza sativa L.). Theor Appl Genet, 2009, 119(3): 459470.

\[14\] Iwata H, Uga Y, Yoshioka Y, et al. Bayesian association mapping of multiple quantitative trait loci and its application to the analysis of genetic variation among Oryza sativa L. germplasm. Theor Appl Genet, 2007, 114(8): 14371449.

\[15\]Eizenga G C, Agrama H A, Lee F N, et al. Identifying novel resistance genes in newly introduced blast resistant rice germplasm. Crop Sci, 2006, 46（5）: 18701878.

\[16\]Agrama H A, Eizenga G C, Yan W. Association mapping of yield and its components in rice cultivars. Mol Breed, 2007, 19（4）: 341356.

\[17\]Huang X H, Wei XH, Sang T, et al. Genomewide association studies of 14 agronomic traits in rice landraces. Genetics 2010, 42（11）: 961970.

\[18\]韩龙植, 张三元.水稻耐冷性鉴定评价方法. 植物遗传资源学报, 2004, 5(1): 7580.

\[19\] Pritchard J K, Stephens M, Donnelly P. Inference of population structure usingmultilocus genotype data. Genetics, 2000, 155: 945959.

\[20\] Edward Buckler Lab. Maize Diversity Research \[20070130\]http://www.maizegenetics.net/ bioinformatics.

\[21\]Breseghello F, Mark E S. Association mapping of kernel size and milling quality in wheat (Triticum aestivum L) cultivars. Genetics, 2006, 172: 11651177.

\[22\]韩龙植, 乔永利, 张媛媛, 等.水稻孕穗期耐冷性QTLs分析. 作物学报, 2005, 31(5): 653657.

\[23\]赵国珍, Yang S J, Yea J D,等.冷水胁迫对云南粳稻育成品种农艺性状的影响. 云南农业大学学报, 2010, （25）3:159163.

\[24\]Tenaillon M, Sawkins M C, Long A D, et al. Pattern of DNA sequence polymorphism along chromosome 1 of maize (Zea mays ssp.mays L.). Proc Natl Acad Sci USA, 2001, 98（16）: 96619666.

\[25\] Zhu Y L, Song Q J, Hyten D, et al. Singlenucleotide polymorphisms in soybean. Genetics, 2003, 163（3）: 11231133.

\[26\]Kraakman A T W, Niks R E, van den Berg P M M M, et al. Linkage disequilibrium mapping of yield and yield stability in modern spring barley cultivars. Genetics, 2004, 168(11): 436446.

\[27\]Garris A J, McCouch S R, Kresovich S K. Population structure and its effect on haplotype diversity and linkage disequilibrium surrounding the xa5 locus of rice (Oryza sativa.). Genetics, 2003, 165(2): 760768.

\[28\] McNally K L, Childs K L,Bohnert R,et al. Genomewide SNP variation reveals relationships among landraces and modern varieties of rice. Proc Natl Acad Sci USA, 2009， 106(30)： 1227312278.

\[29\] Mather K A. The extent of linkage disequilibrium in rice (Oryza sativa L.). Genetics, 2007, 177(4): 22232232.

\[30\]杨小红, 严建兵, 郑艳萍,等. 植物数量性状关联分析研究进展. 作物学报, 2007, 33(4): 523530.

\[31\]乔永利，韩龙植，安永平,等.水稻芽期耐冷性QTL的分子定位. 中国农业科学, 2005, 38(2): 217221.

\[32\] 吴杏春, 王茵, 林文雄.水稻苗期耐冷性状的QTL分析. 中国生态农业学报, 2008,   16(4): 10671069.

\[33\] Lou Q J, Chen L,   Sun Z X, el at. A major QTL associated with cold tolerance at seedling stage in rice (Oryza sativa L). Euphytica, 2007, 158(1/2): 8794.

\[34\]赵正武.水稻越冬耐冷性遗传变异及QTL定位研究\[D\]. 成都：四川农业大学，2006.

\[35\]夏瑞祥.东乡野生稻苗期耐冷性、株高和穗长的QTL分析\[D\]. 扬州：扬州大学，2008.

\[36\] Andaya V C,   Mackill D J. QTLs conferring cold tolerance at the booting stage of rice using recombinant inbred lines from a japonica×indica cross. Thero Appl Genet，2003，106(6): 10841090.

[1]	黄亚茹, 徐鹏, 王乐乐, 贺一哲, 王辉, 柯健, 何海兵, 武立权, 尤翠翠. 外源海藻糖对粳稻品系W1844籽粒灌浆特性及产量形成的影响[J]. 中国水稻科学, 2023, 37(4): 379-391.
[2]	王雨, 孙全翌, 杜海波, 许志文, 吴科霆, 尹力, 冯志明, 胡珂鸣, 陈宗祥, 左示敏. 利用抗稻瘟病基因Pigm和抗纹枯病数量性状基因qSB-9^TQ、qSB-11^HJX改良南粳9108的抗性[J]. 中国水稻科学, 2023, 37(2): 125-132.
[3]	姚姝, 赵春芳, 陈涛, 路凯, 周丽慧, 赵凌, 朱镇, 赵庆勇, 梁文化, 赫磊, 王才林, 张亚东. 低谷蛋白半糯型粳稻营养品质与蒸煮食味品质特征分析[J]. 中国水稻科学, 2023, 37(2): 178-188.
[4]	裴峰, 王广达, 高鹏, 冯志明, 胡珂鸣, 陈宗祥, 陈红旗, 崔傲, 左示敏. 敲除OsNramp5基因创制低镉优质粳稻新材料的应用评价[J]. 中国水稻科学, 2023, 37(1): 16-28.
[5]	陈涛, 赵庆勇, 朱镇, 赵凌, 姚姝, 周丽慧, 赵春芳, 张亚东, 王才林. 利用分子标记辅助选择培育优良食味、低谷蛋白香粳稻新品系[J]. 中国水稻科学, 2023, 37(1): 55-65.
[6]	杨晋宇, 白琛, 丁小惠, 申红芳, 王磊, 应杰政, 鄂志国. 水稻雄性核不育突变体ms7的遗传分析及基因定位[J]. 中国水稻科学, 2022, 36(1): 27-34.
[7]	王才林, 张亚东, 陈涛, 朱镇, 赵庆勇, 姚姝, 赵凌, 赵春芳, 周丽慧, 魏晓东, 路凯, 梁文化. 姊妹系间杂交快速培育优良食味半糯粳稻新品种的育种效果[J]. 中国水稻科学, 2021, 35(5): 455-465.
[8]	王孟佳, 殷敏, 褚光, 刘元辉, 徐春梅, 章秀福, 王丹英, 陈松. 长江中下游双季晚粳稻产量、生育时期及温光资源配置的生态性差异[J]. 中国水稻科学, 2021, 35(5): 475-486.
[9]	王才林, 张亚东, 陈涛, 朱镇, 赵庆勇, 赵春芳, 姚姝, 周丽慧, 赵凌, 魏晓东, 路凯, 梁文化. 地点和播期对半糯粳稻食味品质的影响[J]. 中国水稻科学, 2021, 35(4): 373-382.
[10]	张庆, 胡雅杰, 郭保卫, 张洪程, 徐晓杰, 徐玉峰, 朱邦辉, 徐洁芬, 钮中一, 凃荣文. 太湖地区优良食味高产软米粳稻品种特征研究[J]. 中国水稻科学, 2021, 35(3): 279-290.
[11]	路凯, 陈涛, 姚姝, 梁文化, 魏晓东, 张亚东, 王才林. 盐胁迫下四个水稻类受体蛋白激酶的功能分析[J]. 中国水稻科学, 2021, 35(2): 103-111.
[12]	杨宇尘, 夏原野, 闫志强, 汪磊, 闫秉春, 王镜博, 王祎玮, 栾金华, 徐海. 抽穗开花期喷施茉莉酸甲酯对不同生态区粳稻花时和株型的影响[J]. 中国水稻科学, 2021, 35(1): 69-77.
[13]	王小秋, 杜海波, 陈夕军, 李明友, 王嘉楠, 许志文, 冯志明, 陈宗祥, 左示敏. 江苏近年育成粳稻新品种/系的稻瘟病抗性基因及穗颈瘟抗性分析[J]. 中国水稻科学, 2020, 34(5): 413-424.
[14]	贾琰, 杨亮, 邹德堂, 瞿炤珺, 王敬国, 刘化龙, 王晋, 赵宏伟. 孕穗期冷水胁迫下施用外源物质对寒地粳稻氮光合效率及产量的影响[J]. 中国水稻科学, 2020, 34(5): 443-456.
[15]	魏秀彩, 刘金栋, 刘利成, 黎用朝, 潘孝武, 董铮, 刘文强, 熊海波, 闵军, 李小湘. 基于MAGIC群体的水稻抽穗期和产量相关性状全基因组关联分析[J]. 中国水稻科学, 2020, 34(4): 325-331.